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CLSM imaging Pre-selected rml1 seedlings were mounted on a slide in a drop of water and immediately transferred to a Zeiss confocal microscope LSM780 (Carl Zeiss Microscopy, www.zeiss.de/mikro)

The responsible framing is that the barrier and the immune system are intimately linked, that permeability is a plausible and partly validated contributor, and that the full causal picture is still being worked out

Further, the inherent limitations of studying topical dosing in murine models necessitates further studies of pharmacokinetics and efficacy in larger species, as well as ocular safety and tolerability studies